Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopy

Table 1 Viability of embryos imaged with HGM

Number of embryos	Stage at start of experiment	Duration	Stage at end of experiment	Time interval between imaging (min)	Z levels imaged	Estimated total energy load per embryo	Number developing normally
10	Morula	22 hours	Blastocyst	20	7	4 J	0
12	Morula	22 hours	Blastocyst	20	7	2.5 J	0
8	Morula	22 hours	Blastocyst	60	11	0.9 J	1
8	Morula	24 hours	Blastocyst	not imaged	not imaged	~ 0 J	6
9	Zygote	10 min	Zygote	continuous	10	16 J	6

Embryos were subject to different imaging conditions and their development assessed. From the recipient with embryos subject to 0.9 J of energy, in addition to 1 normal embryo, 3 empty decidua and 2 resorbing embryos were found.

ISSN: 2661-8850