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Table 1 Viability of embryos imaged with HGM

From: Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopy

Number of embryos Stage at start of experiment Duration Stage at end of experiment Time interval between imaging (min) Z levels imaged Estimated total energy load per embryo Number developing normally
10 Morula 22 hours Blastocyst 20 7 4 J 0
12 Morula 22 hours Blastocyst 20 7 2.5 J 0
8 Morula 22 hours Blastocyst 60 11 0.9 J 1
8 Morula 24 hours Blastocyst not imaged not imaged ~ 0 J 6
9 Zygote 10 min Zygote continuous 10 16 J 6
  1. Embryos were subject to different imaging conditions and their development assessed. From the recipient with embryos subject to 0.9 J of energy, in addition to 1 normal embryo, 3 empty decidua and 2 resorbing embryos were found.