Figure 6From: Regulation of endocytic trafficking of transferrin receptor by optineurin and its impairment by a glaucoma-associated mutantThe UBD is required for trafficking of transferrin. HeLa cells infected with adenoviruses expressing shRNA (Ad-shOPTN2) were transfected with shRNA resistant wild type or D474N mutant optineurin. Cells were incubated with Alexa 546-Tfn for 15 min, fixed, stained for optineurin and examined by confocal microscopy.Back to article page