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Figure 8 | BMC Cell Biology

Figure 8

From: Regulation of endocytic trafficking of transferrin receptor by optineurin and its impairment by a glaucoma-associated mutant

Figure 8

The E50K mutant exhibits slower dynamics. (A) Dynamics of optineurin and E50K mutant vesicles. Individual frames from a movie of cells transfected with GFP-optineurin (upper panels) or GFP-E50K (lower panels) with the images acquired every 12 secs. Frame sequence illustrates the movement of a vesicle (denoted by an arrow). Additional files 1 and 2 show the movement of these vesicles. Bar; 5 μm. (B) Velocities of wild type and E50K mutant optineurin vesicles. E50K vesicles move significantly slower compared to wild type optineurin. (C) Pausing time of GFP-optineurin and GFP-E50K vesicles. (D) HeLa cells were transfected with GFP-tagged E50K or wild type optineurin. A representative fluorescence recovery curve after photobleaching is shown. Right panel shows t1/2 time for E50K and optineurin.

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