Figure 2

Adipogenesis, osteogenesis and chondrogenesis of Apodemus ES cells in vitro. Histochemistry and immunofluorescence staining illustrates adipogenesis, osteogenesis and chondrogenesis of Apodemus ES cells. (A) EB outgrowths stained by Oil Red O, (a, b) Alizarin Red, (c, d) or Toluidine Blue (e), EB outgrowths simultaneously stained for positive collagen II expression (f, Red: anti-collagen II antibody; Blue: Hoechst 33342). (B) Representative image of RT-PCR of adipogenesis, osteogenesis and chondrogenesis specific genes, expressed on day 28 of differentiation. Numbers 1, 2 and 3 represent three independent experiments in adipogenesis, osteogenesis and chondrogenesis. Scale bar, 100 μm.