Figure 3

Cardiomyocytes derived from Apodemus ES cell in vitro. Immunofluorescence staining illustrated cardiomyocytes derived from Apodemus ES cells. (A) Cells were stained with anti-desmin (a), anti-sarcomeric α-actinin (d), anti-cardiac myosin heavy chain (g) or anti-cardiac troponin I (j) antibodies as indicated. Hoechst 33342 was used to stain nuclei (b, e, h and k). Figures c, f, i and l are merged images. (B) Expression of cardiac markers (GATA-4 and β-MHC) was determined by RT-PCR. Numbers 1, 2 and 3 represent three independent experiments in cardiomyocyte formation. Scale bar, 100 μm.