Figure 3

Stabilisation of the interaction of RCC1α with chromatin by Ran requires the methylated N-terminal tail of RCC1α. Bar chart showing the mean FRAP t_1/2 +/- SD of live U2OS cells co-expressing wild-type RCC1α-GFP, Δ27RCC1-GFP, RCC1α^K4Q-GFP or RCC1α^D182A-GFP with mCherry empty vector (green), mCherry-Ran^WT (pink) or mCherry-Ran^T24N (red). Data were tested for significant difference in FRAP t_1/2 using the Student's t-test. P-values considered to be statistically different (< 0.001) are indicated as ***.