Figure 4

α-N-terminal methylation of RCC1α is not affected by the stable association of Ran. U2OS cells were co-transfected with GFP, RCC1α-GFP or RCC1α^K4Q-GFP and mCherry, mCherry-Ran^WT or mCherry-Ran^T24N. Cell lysates were analysed by Western blotting for α-dimethylated RCC1, RCC1, Ran and actin as a loading control. Methylated RCC1α-GFP was revealed on a light exposure of the α-dimethylated RCC1 blot and endogenous RCC1 isoforms on a dark exposure. A non-specific reactive band noted by [18] is indicated by *. The migration position of molecular mass markers (kDa) are shown left.