Figure 2

Coordinated Upregulation of IGF2, IGFBP2 and ITGA5 During Osteoblast Differentiation in hMSCs. Adult hMSCs were treated with (black boxes) or without (white boxes) dexamethasone (10^-7M) to induce osteoblastic differentiation and total RNA was analysed using TaqMan Low-Density Array. IGF2, IGFBP2 and ITGA5 mRNA levels were increased by more than 2-fold in dexamethasone-treated MSCs compared to control cells (A-C). This was associated with increased expression of the osteoblast phenotypic genes Runx2, ALP and Col1A1 (D-F). Results are expressed as fold increase compared to untreated cells, after normalization to 18 S expression. The data are representative of two separate experiments using mRNA from two different donors.