From: Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters
Gene | Primer sequence | Number of cycles | Annealing temperature (°C) | Product size(bp) |
---|---|---|---|---|
Insulin | Sense: 5'-GCCTTTGTGAACCAACACCTG -3' Antisense: 5'-GTTGCAGTAGTTCTCCAGCTG -3' | 35 | 67 | 261 |
Glucagon | Sense: 5'-ATCTGGACTCCAGGCGTGCC -3' Antisense: 5'-AGCAATGAATTCCTTGGCAG -3' | 35 | 65 | 170 |
ISL-1 | Sense: 5'-CAACAAACAAAACGCAAAAC -3' Antisense: 5'-AAGTCAAACACAATCCCGA -3' | 35 | 62 | 542 |
PDX-1 | Sense: 5'-GTCCTGGAGGAGCCCAAC -3' Antisense: 5'-GCAGTCCTGCTCAGGCTC -3' | 35 | 65 | 360 |
NeuroD | Sense: 5'-GAAAGCCCTCTGACTGAT -3' Antisense: 5'-AAACTGGCGTGCCTCTAA -3' | 35 | 58 | 314 |
Ngn3 | Sense: 5'-GTAGAAAGGATGACGCCTCAACC-3' Antisense: 5'-TCAGTGCCAACTCGCTCT TAGG-3' | 35 | 62 | 241 |
Nkx2.2 | Sense: 5'-TTCTACGACAGCAGCGACAACC-3' Antisense: 5'-CGTCACCTCCATACCTTTCTG-3' | 35 | 62 | 393 |
Pax-4 | Sense: 5'-GGGGCTCTTTGTGAATGG-3' Antisense: 5'-CCGCAGGACTCGGTTGAT-3' | 35 | 58 | 349 |
Glut-2 | Sense: 5'-CCGCTGAGAAGATTAGAC-3' Antisense: 5'-CTGGATACAGACAGGGAC-3' | 35 | 58 | 492 |
Vimentin | Sense: 5'-CCAGGCAAAGCAGGAGTC-3' Antisense: 5'-GGGTATCAACCAGAGGGAGT-3' | 35 | 62 | 380 |
β-actin | Sense: 5'-CCAAGGCCAACCGCGAGAAGATGAC-3' Antisense: 5'-AGGGTACATGGTGGTGCCGCCAGAC-3' | 35 | 55-67 | 592 |