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Table 1 Primers used in RT-PCR analysis

From: Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters

Gene

Primer sequence

Number of cycles

Annealing temperature (°C)

Product size(bp)

Insulin

Sense: 5'-GCCTTTGTGAACCAACACCTG -3'

Antisense: 5'-GTTGCAGTAGTTCTCCAGCTG -3'

35

67

261

Glucagon

Sense: 5'-ATCTGGACTCCAGGCGTGCC -3'

Antisense: 5'-AGCAATGAATTCCTTGGCAG -3'

35

65

170

ISL-1

Sense: 5'-CAACAAACAAAACGCAAAAC -3'

Antisense: 5'-AAGTCAAACACAATCCCGA -3'

35

62

542

PDX-1

Sense: 5'-GTCCTGGAGGAGCCCAAC -3'

Antisense: 5'-GCAGTCCTGCTCAGGCTC -3'

35

65

360

NeuroD

Sense: 5'-GAAAGCCCTCTGACTGAT -3'

Antisense: 5'-AAACTGGCGTGCCTCTAA -3'

35

58

314

Ngn3

Sense: 5'-GTAGAAAGGATGACGCCTCAACC-3'

Antisense: 5'-TCAGTGCCAACTCGCTCT TAGG-3'

35

62

241

Nkx2.2

Sense: 5'-TTCTACGACAGCAGCGACAACC-3'

Antisense: 5'-CGTCACCTCCATACCTTTCTG-3'

35

62

393

Pax-4

Sense: 5'-GGGGCTCTTTGTGAATGG-3'

Antisense: 5'-CCGCAGGACTCGGTTGAT-3'

35

58

349

Glut-2

Sense: 5'-CCGCTGAGAAGATTAGAC-3'

Antisense: 5'-CTGGATACAGACAGGGAC-3'

35

58

492

Vimentin

Sense: 5'-CCAGGCAAAGCAGGAGTC-3'

Antisense: 5'-GGGTATCAACCAGAGGGAGT-3'

35

62

380

β-actin

Sense: 5'-CCAAGGCCAACCGCGAGAAGATGAC-3'

Antisense: 5'-AGGGTACATGGTGGTGCCGCCAGAC-3'

35

55-67

592

  1. Abbreviations: ISL-1, islet-1 transcription factor; PDX-1, pancreatic duodenal homeobox-1; NeuroD, neurogenic differentiation; Ngn3, neurogenin 3; Nkx2.2, NK-2 family homeodomain transcription factor; Pax-4, paired box gene 4; Glut-2, glucose transporter 2.