Skip to main content

Table 1 Primers used in RT-PCR analysis

From: Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters

Gene Primer sequence Number of cycles Annealing temperature (°C) Product size(bp)
Insulin Sense: 5'-GCCTTTGTGAACCAACACCTG -3'
Antisense: 5'-GTTGCAGTAGTTCTCCAGCTG -3'
35 67 261
Glucagon Sense: 5'-ATCTGGACTCCAGGCGTGCC -3'
Antisense: 5'-AGCAATGAATTCCTTGGCAG -3'
35 65 170
ISL-1 Sense: 5'-CAACAAACAAAACGCAAAAC -3'
Antisense: 5'-AAGTCAAACACAATCCCGA -3'
35 62 542
PDX-1 Sense: 5'-GTCCTGGAGGAGCCCAAC -3'
Antisense: 5'-GCAGTCCTGCTCAGGCTC -3'
35 65 360
NeuroD Sense: 5'-GAAAGCCCTCTGACTGAT -3'
Antisense: 5'-AAACTGGCGTGCCTCTAA -3'
35 58 314
Ngn3 Sense: 5'-GTAGAAAGGATGACGCCTCAACC-3'
Antisense: 5'-TCAGTGCCAACTCGCTCT TAGG-3'
35 62 241
Nkx2.2 Sense: 5'-TTCTACGACAGCAGCGACAACC-3'
Antisense: 5'-CGTCACCTCCATACCTTTCTG-3'
35 62 393
Pax-4 Sense: 5'-GGGGCTCTTTGTGAATGG-3'
Antisense: 5'-CCGCAGGACTCGGTTGAT-3'
35 58 349
Glut-2 Sense: 5'-CCGCTGAGAAGATTAGAC-3'
Antisense: 5'-CTGGATACAGACAGGGAC-3'
35 58 492
Vimentin Sense: 5'-CCAGGCAAAGCAGGAGTC-3'
Antisense: 5'-GGGTATCAACCAGAGGGAGT-3'
35 62 380
β-actin Sense: 5'-CCAAGGCCAACCGCGAGAAGATGAC-3'
Antisense: 5'-AGGGTACATGGTGGTGCCGCCAGAC-3'
35 55-67 592
  1. Abbreviations: ISL-1, islet-1 transcription factor; PDX-1, pancreatic duodenal homeobox-1; NeuroD, neurogenic differentiation; Ngn3, neurogenin 3; Nkx2.2, NK-2 family homeodomain transcription factor; Pax-4, paired box gene 4; Glut-2, glucose transporter 2.