Figure 3
From: Yeast karyopherin Kap95 is required for cell cycle progression at Start
Subcellular localization of Swi6 in the kap95 mutant strain. Cells from exponentially growing cultures of the wild type (JCY710) and tetO 7 :KAP95 (JCY637) strains expressing the HA-tagged version of Swi6, were incubated in the presence of 5 μg/mL doxycycline for 6 hours and assayed by indirect immunofluorescence. The HA indirect-fluorescence signals, the DAPI staining of DNA and DIC images are shown. No signal was detected in a control of the untagged W303-1a strain.