Figure 2

Identification of the minimal Ror1 domain that plays a critical role in nuclear accumulation. Confocal microscopic analysis was used to detect subcellular localization of Ror1 fragments. HR cells were transfected for 24 hours with indicated Ror1 fragments and stained with anti-HA monoclonal antibody (green) followed by a fluorescent (FITC) secondary antibody and with phalloidin rhodamine (red). DAPI staining shows the nucleus. PKM2, a known cytoplasmic protein, served as a negative control.