Heterogeneity of mouse EPCs immediately after isolation and EPC subclones are bipotent. A-B) CD31+ BECs surround LECs, which are in the centre of a growing colony and which are Lyve1 positive. BECs are more positive for CD31 than LECs and all LECs specifically express the transcription factor Prox1. C-D) After a single-cell clonogenic assay all cells from two representative subclones (D5 and B3) are positive for CD31, a panendothelial marker. Only cells differentiated into lymph endothelial cells (LECs) are Lyve1 or podoplanin positive. 150-200-fold.