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Figure 2 | BMC Cell Biology

Figure 2

From: The FPR2-induced rise in cytosolic calcium in human neutrophils relies on an emptying of intracellular calcium stores and is inhibited by a gelsolin-derived PIP2-binding peptide

Figure 2

The fMLF and WKYMVM peptides induce increases in intracellular calcium in neutrophils. Neutrophils loaded with Fura-2 were incubated at 37°C for 5 minutes. PBP10 (10-6 M final concentration) was present for the initial 5 minutes (B and D), and the cells were stimulated with fMLF (A and B; 10-7 M final concentration) or WKYMVM (C and D; 10-7 M final concentration). The changes in cytosolic Ca2+ levels were determined by measuring the fluorescence emitted at 509 nm following excitation at 340 nm and 380 nm. The results are presented as the ratios of the fluorescence intensities at 340 nm and 380 nm, and a representative experiment (of at least five repetitions) is shown.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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