Figure 3
The rise in intracellular calcium induced by low concentrations of WKYMVM in neutrophils is inhibited by PBP10. Neutrophils loaded with Fura-2 were incubated at 37°C for 5 minutes. PBP10 (10-6 M final concentration, broken lines) was present for the initial 5 minutes (control cells were incubated without PBP10, solid lines), and the cells were stimulated with decreasing concentrations of fMLF (upper part of the figure) or WKYMVM (lower part of the figure). The changes in cytosolic Ca2+ levels were determined by measuring the fluorescence emitted at 509 nm following excitation at 340 nm and 380 nm. A representative experiment of at least five performed is shown.