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Figure 5 | BMC Cell Biology

Figure 5

From: The FPR2-induced rise in cytosolic calcium in human neutrophils relies on an emptying of intracellular calcium stores and is inhibited by a gelsolin-derived PIP2-binding peptide

Figure 5

Effects of increasing concentrations of EGTA on ionomycin-induced production of superoxide anions by neutrophils. Neutrophils were stimulated with ionomycin (5 × 10-7 M final concentration) without or with increasing concentrations of the Ca2+ chelator EGTA. EGTA was added to the samples 20 seconds before the addition of the ionophore (time 0). Intracellular production of superoxide was assessed using a luminol-amplified chemiluminescence technique.

Light emission was measured with or without EGTA, and a representative experiment of at least five performed is shown. Abscissa, time of study (minutes); ordinate, superoxide production, given as light emission and expressed as cpm × 10-6.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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