Figure 3

Arecoline effects on expression of tight junction and adherens junction proteins in Ishikawa cells. Ishikawa cells were treated with DMSO (vehicle control) or with either 0.3 mM or 0.5 mM arecoline for 48 hrs, and total cell extracts were fractionated in SDS polyacrylamide gels. The production of ZO-1, Claudin-1, E-cadherin, and beta-catenin protein was determined by western blot analysis and compared to the levels of actin protein.