Figure 5From: Arecoline induced disruption of expression and localization of the tight junctional protein ZO-1 is dependent on the HER 2 expression in human endometrial Ishikawa cellsImmunofluorescence analyiss of arecoline effects on the cellular staining pattern of E-cadherin. Ishikawa cells were treated with the DMSO vehicle control or with either 0.3 mM, or 0.5 mM arecoline for 48 hrs. Cells were fixed in 3.7% formaldehyde and stained for localization of E-cadherin by indirect immunofluorescence and for nuclear DNA by DAPI staining. Bars = 20 μm.Back to article page