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Figure 6 | BMC Cell Biology

Figure 6

From: The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: Methodology

Figure 6

Cell retention. Box plot of the percentage of dislodging cells during the freezing - thawing cycle. U937 cells were loaded into the i3C (n = 5), and the same area (20 × 20 picowells) was imaged in the course of cell handling. Total cells dislodging was calculated after each step: 1 - after pre-cooling, 2 - immediately after thawing, 3 - post-thawing after opening the cryostage lid, 4 - after post-thawing medium exchange. The mean value for each data set is indicated by the central line and the first and third quartiles are the edges of the box area.

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