Figure 4

Confocal microscopic analysis of FMNL2 in WM164 melanoma cells and A431 squamous cell carcinoma cells. FMNL2 (left, red) was visualized with antibody, F-actin (middle, green) with phalloidin, the nucleus with DAPI (blue) and the cells analyzed with a confocal microsope. Top row: In WM164 cells, FMNL2 is located both diffusely in the cytoplasm and perinuclearly (blue) consistent with the location of the Golgi apparatus (arrowhead). WM164 cells have prominent protrusions with long actin filaments. At the tips of these protrusions, FMNL2 and F-actin are co-localized (arrows). Bottom row: A431 cells lack protrusions. FMNL2 is seen in the cytoplasm, faintly concentrated to the perinuclear location (arrowhead). No co-localization with F-actin is evident. Bar = 20 μm.