Figure 4

H ₂ O ₂ provoked DNA-PK and JNK activity. (A) InR1-G9 cells were treated with Zeocin (50, 100 or 200 μg/ml) for indicated time before harvested. Oct-1 expression in the nuclear extract (NE) was then assessed. (B) InR1-G9 cells were treated with vehicle, Zeocin (100 μg/ml) or H₂O₂ (00 μM) for 2 h before harvested for DNA-PK assay. The values are mean +/- S.E (n = 3). *, P < 0.05. (C, D) InR1-G9 cells were treated with forskolin/IBMX (C) or H₂O₂ (100 μM) (D) for indicated time. Whole cell lysates were utilized for assessing the expression of JNK and phosphorylated JNK, ERK and phosphorylated ERK.