Maged1 is induced early during myogenic differentiation. (A) Maged1, p21CIP1/WAF1 and MyoG RNA levels were determined using qRT-PCR during the differentiation of C2C12 myoblasts induced by serum starvation. RNA levels were normalized using GAPDH RNA as the reference. Data are presented as the relative folds of induction at different time points with respect to the level measured at the time of serum starvation (0 h). (B-E) Immunoblotting analysis of Maged1 in C2C12 myoblasts (B) and primary mouse myoblasts (C) during myogenic differentiation and in TA muscle regenerating after CTX injection in wild-type mice (D) and in Maged1tm1Urfm/HSA-Cre mice that have a cell-type specific deletion of Maged1 in myoblasts (E). Induction of Maged1 was quantified by digital imaging using alpha-tubulin for normalization (B and C).