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Figure 2 | BMC Cell Biology

Figure 2

From: Peptide aptamers as new tools to modulate clathrin-mediated internalisation — inhibition of MT1-MMP internalisation

Figure 2

GFP-swiggle co-immunoprecipitates with MT1-MMP. (A) Western blot analysis of protein extracts prepared from MCF7 expressing GFP (lane 1), GFP-TrxA (lane 2), GFP-s14 (lane 3) or GFP-swiggle (lane 4) using an anti-GFP polyclonal serum. (B) Cell lysates prepared from MCF7 cells expressing MT1-MMP and GFP-TrxA (lanes 1 and 3), MT1-MMP and GFP-swiggle (lanes 2 and 4) were immunoprecipitated with an anti-GFP polyclonal antibody (lanes 3 and 4). The presence of MT1-MMP (top panel) and GFP-TRxA and GFP-swiggle (middle panel) in the immunocomplexes was monitored by Western blotting. The expression level of MT1-MMP in the input lysates was analysed by Western blot (bottom panel). (C) Cell lysates from MCF7 cells expressing MT1-MMP (lanes 1 and 2), MT1-MMP and GFP-s14 (lanes 3 and 5), MT1-MMP and GFP-swiggle (lanes 4 and 6) were immunoprecipitated with an anti-MT1-MMP pAb (lanes 1, 3 and 4). MT1-MMP, GFP-s14 or GFP-swiggle were detected in the input lysates (lanes 2, 5 and 6) and immunoprecipitated materials (lanes 1, 3 and 4) using an anti-MT1-MMP pAb or an anti-GFP polyclonal serum, respectively.

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