Figure 7
Quantification of MT1-MMP endocytosis in MCF7 cells transfected with MT1-MMP and GFP-s14 or GFP-swiggle. MCF7 cells transiently co-transfected with MT1-MMP and either GFP-s14 or GFP-swiggle were incubated with an anti-MT1-MMP antibody for 2 hours at 4°C. Cells were fixed immediately (0 min) or after 10, 30 or 50 minutes at 37°C. Data represent the average of the total fluorescence intensity of endosomes per cell ± s.e.m..