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Figure 3 | BMC Cell Biology

Figure 3

From: Karyopherin α7 (KPNA7), a divergent member of the importin α family of nuclear import receptors

Figure 3

KPNA7 contains an N-terminal IBB. (A) KPNA proteins were translated as 35S-methionine labeled polypeptides and used in binding assays with GST-Importin β. (B) Full-length and ΔIBB forms of KPNA7 tested for binding to Importin β. (C) Importin β binding to the IBB of KPNA7 is RanGTP-sensitive. Recombinant Importin β binding to GST-IBB proteins was performed in the absence and presence of recombinant RanGTP, and by subsequent addition of RanGTP to preformed GST-IBB-importin β complexes. These assays were performed with the GTPase deficient RanQ69L mutant. Bound and eluted fractions were examined by immunoblotting using a monoclonal antibody to Importin β. (D) KPNA1, KPNA2, KPNA7, KPNA7 ΔIBB binding to GST-SV40 NLS using 35S-methionine labeled polypeptides.

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