Figure 2

No differences in mRNA levels between WT and A267T PC. CHO-K1 (A) and Huh7 cells (B) were transiently transfected with plasmid constructs expressing WT or A267T PC. Cells were harvested 24 h after transfection and total RNA was isolated. PC mRNA levels were determined using quantitative RT-PCR with 18 S and GAPDH as endogenous controls for CHO-K1 and Huh7 cells, respectively. The mRNA PC levels i Huh7 cells were normalized for the endogenous mRNA PC levels. The results are presented as ratio between mRNA levels of WT and A267T PC with the WT PC level assigned as 1. The results are from three independent transfection experiments performed in triplicates and presented as mean ± SEM.