Intracellular localization of WT and A267T PC. CHO-K1 cells were grown on coverslips and transiently transfected with plasmid constructs expressing WT or A267T PC. Forty-eight h after transfection the cells were fixed and stained with anti-PC and anti-PDI (ER marker) or anti-GM130 (Golgi marker). PC appears red, PDI and GM130 appears green and overlaid images is yellow and corresponds to areas of co-localization of PC with ER and Golgi. Scale bar, 10 μm.