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Table 1 Effect of protein degradation inhibitors on intracellular PC levels.

From: Functional characterization of the protein C A267T mutation: evidence for impaired secretion due to defective intracellular transport

Treatments Intracellular WT PC (%) Intracellular A267T PC (%)
Control 100 100
Lactacystin (10 μM), n = 8 119.8 ± 3.3* 131.8 ± 4.4*
Bafilomycin (100 nM), n = 9 112.6 ± 4.9 106.7 ± 7.2
  1. Transient transfected CHO-K1 cells expressing WT or A267T PC were incubated with medium containing 10 μM lactacystin or 100 nM bafilomycin for 24 and 8 h, respectively. PC protein levels were measured in cell lysates and results are calculated as percentage of controls (samples not treated with protein degradation inhibitors). Data are presented as mean ± SEM. *p < 0.05 (Wilcoxon test).
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