|
Treatments
|
Intracellular WT PC (%)
|
Intracellular A267T PC (%)
|
|---|
|
Control
|
100
|
100
|
|
Lactacystin (10 μM), n = 8
|
119.8 ± 3.3*
|
131.8 ± 4.4*
|
|
Bafilomycin (100 nM), n = 9
|
112.6 ± 4.9
|
106.7 ± 7.2
|
- Transient transfected CHO-K1 cells expressing WT or A267T PC were incubated with medium containing 10 μM lactacystin or 100 nM bafilomycin for 24 and 8 h, respectively. PC protein levels were measured in cell lysates and results are calculated as percentage of controls (samples not treated with protein degradation inhibitors). Data are presented as mean ± SEM. *p < 0.05 (Wilcoxon test).
