Figure 6

cAMP-induced formation of junction strands is independent of PKA. HUVEC were pretreated with H-89 and exposed to the different cAMP derivates for (A) 24 h or (B) 72 h. Cells were fixed and double stainings for ZO-1 (green) and CLDN5 (red) were performed. Shown are representative micrographs of three independent experiments. Scale bar represents 25 μm.