Effect of cAMP derivates on expression and localization of TRIC. (A) pCPT-cAMP, 8-Br-cAMP/Na and cAMP induced increased expression of TRIC mRNA as determined by real-time qPCR. Expression data were normalized to expression of HPRT-1 (n = 3). (B) pCPT-cAMP and 8-Br-cAMP/Na induced a faint membrane translocation of TRIC after 24 h. After 72 h 8-Br-cAMP/Na-stimulated cells showed rather strong membrane localization of TRIC whereas pCPT-cAMP led to only moderate TRIC staining (n = 4). The nucleus was counterstained with DAPI. Scale bar represents 25 μm.