Localization and FRET interactions of endogenous ARE binding proteins upon MAPK activation. ARE-BPs were detected via immunocytochemistry. Cells were treated with 75 nM anisomycin for 24 hours. The localization of the ARE-BPs is shown in control cells (top row), and anisomycin treated cells (bottom row), in each panel of images. Images in each row, from left to right, show the localization of an ARE-BP detected via: i) a Cy3 labeled 2° Ab in green, ii) a Cy5 or AF647 labeled 2° Ab in red, iii) merged Cy3-Cy5 image, iv) FRETc signal in a thermal pseudocolor scale. A color matched signal intensity scale is indicated for each image. All scale bars are 10 μm in length. Cell outline as shown. Panel A: HuR (Cy3) and AUF1 (Cy5). Panel B: KSRP (Cy3) and HuR (Cy5). Panel C: HuR (Cy3) and TIA-1 (AF647). Panel D: KSRP (Cy3) and TIA-1 (AF647). Panel E: AUF1 (Cy3) and TIA-1 (AF647). Panel F: KSRP (Cy3) and AUF1 (Cy5).