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Figure 5 | BMC Cell Biology

Figure 5

From: Intracellular localization and interaction of mRNA binding proteins as detected by FRET

Figure 5

Localization and FRET interactions of exogenous ARE binding proteins upon MAPK activation. ARE binding proteins were detected via transient transfection of labeled vector constructs into DDT1-MF2 cells. Cells were treated with 75 nM anisomycin for 24 hours. The localization of ARE-BPs is shown in control cells (top row), and anisomycin treated cells (bottom row), in each panel of images. Images in each row, from left to right, show the localization of an ARE-BP detected via: i) YFP in green, ii) CFP in blue, iii) merged YFP and CFP images, iv) FRETc signal in a thermal pseudocolor scale. A color matched signal intensity scale is indicated for each image. All scale bars are 10 μm in length. Cell outline as shown. Panel A: HuR-YFP and p37AUF1-CFP. Panel B: HuR-YFP and KSRP-CFP. Panel C: TIA-1-YFP and HuR-CFP. Panel D: TIA-1-YFP and KSRP-CFP. Panel E: TIA-1-YFP and p37AUF1-CFP. Panel F: p37AUF1-YFP and KSRP-CFP.

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