Skip to main content
Figure 7 | BMC Cell Biology

Figure 7

From: Intracellular localization and interaction of mRNA binding proteins as detected by FRET

Figure 7

Interaction of Processing body components Hedls and Dcp1a with ARE containing mRNA. 2'F stabilized, Cy3-labeled β-AR mRNA was transiently transfected into DDT1-MF2 cells. The cells were subjected to oxidative stress (30 minute exposure to 0.5 mM sodium arsenite (SA)) and the proteins were detected by immunocytochemistry. Top row, control cells, bottom row, SA treated cells. Images in each row, from left to right, show the localization of: i) the Cy3-labeled mRNA in red, ii) a Cy5-labeled 2° Ab in blue, iii) HuR detected via an AF488 labeled 2° Ab in green, iv) merged Cy3, Cy5 and AF488 image, v) Cy3-Cy5 FRETc signal in a thermal pseudocolor scale. A color matched signal intensity scale is indicated for each image. All scale bars are 10 μm in length. Cell outline as shown. Panel A: β-AR mRNA (Cy3), Hedls (Cy5), and HuR (AF488). The inset in the merged view shows a close up of the SGs (green) and PBs (blue) interaction. No FRETc image presented due to the disparity of the Cy3/Cy5 signal intensity. Panel B: β-AR mRNA (Cy3), Dcp1a (Cy5), and HuR (AF488). The inset in the merged view shows the interaction between SGs (green) and PBs (blue).

Back to article page