Spindle assembly checkpoint genes reveal distinct as well as overlapping expression that implicates MDF-2/Mad2 in postembryonic seam cell proliferation in Caenorhabditis elegans

Table 2 The average number of SCM::GFP nuclei is altered in mdf-2(tm2190) mutants

Genotype	Hypodermal seam-cell nuclei (n)
	L1	L2	L3	L4	Adult
Wild type	10.02 ± 0.08 (48)	15.76 ± 0.12 (25)	15.96 ± 0.04 (25)	16.04 ± 0.09 (25)	16.00 ± 0.08 (49)
mdf-2(tm2190)	9.75 ± 0.09* (59)	14.36 ± 0.30* (25)	14.08 ± 0.25* (25)	14.20 ± 0.39* (25)	14.28 ± 0.17* (64)

All of the strains are homozygous for the seam cell marker wIs51 (SCM::GFP). The number of SCM::GFP positive nuclei was scored 0 h after hatching (L1), two days after hatching (L4) and 12 h after L4 stage (young adults). Asterisk denotes significant difference when compared to wild type using unpaired students t-test analysis (p = 0.012 for L1 stage; p = 7.21E-5 for L2 stage; p = 2.57E-10 for L3 stage; p = 3.35E-5 for L4 stage and p = 2.96E-12 for adult stage.

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