Figure 4

Effects of CTDSPL2 repression on globin gene expression in K562 cells. (A) Fluorescent microscopy indicating CTDSPL2-GFP expression showing CTDSPL2 repression by two siRNAs in Hela cells. (B) Real-time PCR analysis of CTDSPL2 mRNA expression in stable K562/CTDSPL2 RNAi transfectants. (C) Hemoglobin-containing cells were detected by benzidine staining during hemin-induced erythroid differentiation of the above K562 cells. The data were obtained from three independent experiments and the error bars represent standard deviation. (D) Real-time PCR analysis of α-, ζ-, ε-, γ- and β-globin mRNA expression in the stable K562/CTDSPL2RNAi transfectants carrying the recombinant pLVTHM CTDSPL2RNAi (RNAi) and the stable K562/CTDSPL2 transfectants carrying the blank pLVTHM (Control). (E) Real-time PCR analysis of α-, ζ-, ε-, γ- and β-globin mRNA expression 72 h after hemin treatment in stable K562/CTDSPL2RNAi transfectants and the stable K562/CTDSPL2 transfectants.