Skip to main content

Table 1 The expression levels of "stemness" genes and differentiation markers.

From: A feeder-free culture using autogeneic conditioned medium for undifferentiated growth of human embryonic stem cells: Comparative expression profiles of mRNAs, microRNAs and proteins among different feeders and conditioned media

A. 21"stemness" genes

     

Probe ID

Gene Symbol

T3/HDF

T3/CMHDF

T3/MEF

T3/CMMEF

UniGene

Gene Description

206286_s_at

TDGF1

638.00

555.60

325.10

352.70

Hs.385870

Teratocarcinoma-derived growth factor 1, CRIPTO

220184_at

NANOG

586.80

674.20

614.80

680.70

Hs.329296

Nanog homeobox

210905_x_at

ASH1L

331.30

335.60

196.10

197.80

Hs.491060

ASH1L (Drosophila), hypothetical protein, 2969aa

210265_x_at

POU5F1L

186.10

176.50

236.80

221.70

Hs.504658

Similar to POU domain Class 5 transcription factor 1

219651_at

DPPA4

126.40

137.90

92.07

116.50

Hs.317659

Developmental pluripotency associated 4, 294aa

224048_at

USP44

94.35

81.71

43.63

44.44

Hs.506394

Ubiquitin specific protease 44

229661_at

SALL4

87.55

15.74

4.45

2.93

Hs.517113

Sal-like 4 (Drosophila), zinc finger protein

208286_x_at

POU5F1

87.31

79.01

86.01

92.14

Hs.249184

POU domain Class 5 Transcription Factor 1,OCT4, 360aa

206309_at

LECT1

66.61

62.77

25.02

38.87

Hs.421391

Leukocyte cell derived chemotaxin 1

214532_x_at

POU5F1P1

65.41

63.29

47.31

56.49

Hs.450254

POU domain, OCT4-related intron-less gene, 359aa

240301_at

DPPA2

55.90

119.20

27.86

43.06

Hs.351113

Developmental pluripotency associated 2, 221aa

206023_at

NMU

54.32

91.65

44.98

40.92

Hs.418367

Neuromedin U

214829_at

AASS

30.42

21.40

7.60

9.77

Hs.528295

Aminoadipate-semialdehyde synthase

213050_at

COBL

23.24

19.65

3.35

5.52

Hs.99141

Cordon-bleu homolog (mouse)

208542_x_at

ZNF208

14.12

10.08

2.94

3.97

Hs.419763

Zinc finger protein 208

231698_at

UGP2

8.20

12.80

8.31

10.50

Hs.516217

UDP-glucose pyrophosphorylase 2

205309_at

SMPDL3B

5.88

2.90

4.68

2.95

Hs.123659

Acid sphingomyelinase-like phosphodiesterase

202889_x_at

MAP7

5.44

6.46

5.38

5.18

Hs.486548

Microtubule-associated protein 7

230623_x_at

USP28

5.12

8.38

2.81

4.55

Hs.503891

Ubiquitin specific protease 28

215509_s_at

BUB1

4.90

2.29

3.45

2.33

Hs.469649

BUB1 budding (yeast), human spindle check point kinase

207199_at

TERT

4.03

4.06

3.27

2.24

Hs.492203

Telomerase reverse transcriptase

B. 9 differentiation markers

     

Probe ID

Gene Symbol

T3/HDF

T3/CMHDF

T3/MEF

T3/CMMEF

UniGene

Gene Description

Ectoderm

       

225540_at

MAP2

0.12

0.13

0.59

0.61

Hs.368281

microtubule-associated protein 2

1556057_s_at

NEUROD1

0.54

0.56

0.67

0.68

Hs.709709

neurogenic differentiation 1

1555938_x_at

VIM

0.17

0.19

0.52

0.40

Hs.642813

vimentin

Mesoderm

       

205932_s_at

MSX1

0.09

0.07

0.10

0.07

Hs.424414

msh homeobox 1

202222_s_at

DES

0.63

0.67

0.68

0.63

Hs.594952

desmin

1556499_s_at

COL1A1

0.49

0.19

0.20

0.34

Hs.172928

collagen, type I, alpha 1

Endoderm

       

224646_x_at

H19

0.58

0.75

0.97

1.00

Hs.533566

H19, imprinted maternally untranslated mRNA

214701_s_at

FN1

0.08

0.07

0.41

0.16

Hs.203717

fibronectin 1

211896_s_at

DCN

0.01

0.02

0.01

0.03

Hs.706840

decorin