Figure 2

Expression of GDNF and its receptor subunits in cultured ISCs and TM4 cells. (A) RT-PCR results exhibited the expression of GDNF, GFRα1 and NCAM but not RET mRNAs in cultured ISCs and TM4 cells. G3PDH and the Sertoli cell-specific gene CLU were used as positive controls. (B) RET expression in SSCs but not cultured ISCs or TM4 cells was detected by RT-PCR. (C-E) Western blotting demonstrated the expression of NCAM (C) and GFRα1 (D) but not RET (E) proteins in cultured ISCs and TM4 cells. The SY5Y cell line was used as the positive control for the RET protein. The β-actin protein was used as a positive control for each sample.