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Figure 4 | BMC Cell Biology

Figure 4

From: GDNF stimulates the proliferation of cultured mouse immature Sertoli cells via its receptor subunit NCAM and ERK1/2 signaling pathway

Figure 4

Phosphorylation of ERK1/2 but not AKT in ISCs is upregulated by GDNF. (A) Time-course of ERK1/2 phosphorylation levels in cultured ISCs stimulated with GDNF and the first bar (0) is cells without GDNF treatment. Total ERK1/2 was the internal control in the semi-quantitative densitometry analysis (right panel). (B) Time course of AKT phosphorylation levels in ISCs stimulated with GDNF, with total AKT as an internal control in the semi-quantitative densitometry analysis (right panel). (C) Time course of ERK1/2 phosphorylation level in PD98059 pre-treated (10 μM) ISCs stimulated with GDNF and the bar (control) is cells without GDNF or PD98059 treatment, with total ERK1/2 as the internal control in the semi-quantitative densitometry analysis (right panel). (D) Time course of AKT phosphorylation levels in PD98059 pre-treated (10 μM) ISCs stimulated with GDNF, with total AKT as the internal control in the semi-quantitative densitometry analysis (right panel). The data were presented as means ± SD from three independent experiments. Statistically significant differences (p < 0.05) among groups are indicated by an asterisk.

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