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Figure 4 | BMC Cell Biology

Figure 4

From: PIST regulates the intracellular trafficking and plasma membrane expression of Cadherin 23

Figure 4

PIST reduced the membrane expression of cadherin 23 in transfected HEK293 cells. A. Myc-cadherin 23 immunoreactivity using a non-permeable protocol shows clear plasma membrane localization. B. In the presence of EGFP-PIST, Myc-cadherin 23's plasma membrane immunoreactivity is undetectable. Staining was performed as normal except Triton X-100 was excluded. Myc-cadherin 23 was stained with mouse anti-Myc antibody and then Cy5-conjugated goat anti-mouse secondary antibody. mCherry was used as a control indicating Myc-cadherin 23-IRES-mCherry cassette was expressed successfully. Scale bar, 10 μm.

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