Figure 6
From: PIST regulates the intracellular trafficking and plasma membrane expression of Cadherin 23
MAGI-1 and harmonin release cadherin 23 from PIST's retention in transfected HEK293 cells. A. Myc-tagged cadherin 23 co-localizes with HA-tagged PIST at TGN-like structures. B. In the presence of EGFP-MAGI-1c, Myc-cadherin 23 colocalizes in the cytoplasm with EGFP-MAGI-1c, instead of co-localizing with HA-PIST at TGN-like structures. C. In the presence of EGFP-harmonin, Myc-cadherin 23(-68) co-localizes with EGFP-harmonin, instead of co-localizing with HA-PIST at TGN-like structures. D. In the presence of EGFP-harmonin, Myc-cadherin 23(+68) co-localizes with EGFP-harmonin, as well as with HA-PIST at TGN-like structures. Myc-cadherin 23 was stained with mouse anti-Myc antibody then TRITC-conjugated goat anti-mouse secondary antibody. HA-PIST was stained with rabbit anti-PIST antibody then Cy5-conjugated goat anti-rabbit secondary antibody. Scale bar, 10 μm.