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Figure 5 | BMC Cell Biology

Figure 5

From: The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. II: Functional activity of cryopreserved cells

Figure 5

Monitoring U937 cell membrane integrity after the freezing - thawing cycle by FDA staining. The U937 cells were exposed to the freezing - thawing cycle in the i3C. At 3 hours after thawing, FDA solution (1.2 μM) was introduced into the i3C and images were taken 5 minutes after FDA addition. The transmitted image (A) and corresponding fluorescent image (B) are presented. The dead U937 cell unstained by FDA is indicated by the red arrow. Scale bar: 0.01 mm.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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