Skip to main content

Table 1 Cell death in U937 cells after the freezing - thawing cycle.

From: The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. II: Functional activity of cryopreserved cells

Time after thawing

The devices used for FTC

 

i3C

mini-vial

Standard cryo-vial

   

2 × 106cells/ml

10 × 106cells/ml

1 hour (after-80°C)

8.7% ± 3.5

 

9.1% ± 5.6

 

3 hours (after-80°C)

9.2% ± 5.1

6.3% ± 3.2

12.5% ± 4.1

 

overnight (after-80°C)

23.4% ± 23.5

11.6% ± 2.3

19.6% ± 10.1

17.5% ± 11.3

overnight (after-196°C)

18.5 ± 2.9

 

24.6 ± 7.0

 
  1. U937 cells underwent a freezing - thawing cycle as described in Materials and Methods. The percentage of dead (PI positive) cells was tested at appropriate time points after thawing. The corresponding temperature end points are presented. Values are average ± SD of 2-3 experiments (500-1000 individual cells).