Figure 4
From: Mcm10 proteolysis initiates before the onset of M-phase
Mcm10 is present in most cells that were positive for cyclin A. (A) A field of asynchronously growing HeLa cells visualized for Mcm10 and cyclin A. Mcm10 was visualized by a rabbit anti-Mcm10 antibody in conjugation with anti-rabbit FITC antibody (third panel) while cyclin A was visualized with mouse anti-cyclin A antibody in conjugation with anti-mouse Alexa 594 antibody (first panel). DNA was stained with DAPI (second panel) while the right panel is a merge of images obtained from FITC, Alexa 594 and DAPI immunofluorescence. Cell 1 is positive for FITC and negative for Alexa 594 signal while cell 2 is positive for Alexa 594 but negative for FITC signal and cell 3 is positive for both demonstrating that there is no bleed-through of fluorescent signals. (B) RNAi confirms the cyclin A immunofluorescence signal while Mcm10 signal has been authenticated previously [11]. HeLa cells were transfected with GL2 or CYCLIN A siRNA oligos and later processed for immunofluorescence with the mouse anti-cyclin A antibody. The scale bar is 20 microns.