Figure 2

The ASPM spindle association is microtubule-dependent. A. Panel showing a nocodazole-treated mitotic HeLa cell immunostained for ASPM 216-1 (green), γ-tubulin (red) and DAPI (blue). ASPM is not clustered around the γ-tubulin of the spindle poles in the absence of microtubules. Scale bar = 5 μm. B. Panel showing a taxol-treated mitotic HeLa cell immunostained for ASPM 216-1 (green), α-tubulin (red) and DAPI (blue). ASPM concentrates in the centre of taxol-induced microtubule asters (arrows). Scale bar = 5 μm. C. Panel showing a taxol-treated mitotic HeLa cell immunostained for ASPM 216-1 (green), γ-tubulin (red) and DAPI (blue). ASPM (arrowhead) does not specifically co-localize with γ-tubulin foci (arrow) in taxol-treated cells. Scale bar = 5 μm.