Figure 5

Influence of methylprednisolone and hydroxychloroquine on intracellular localization of SP-C^WT or SP-C^I73T. Stabile MLE-12 cells expressing SP-C^WT or SP-C^I73T were incubated 24 hours with 10 μM of methylprednisolone (+Met) or hydroxychloroquine (+Hyd) and analyzed by immunofluorescence followed by quantification of colocalization or proSP-C (HA-tag) with syntaxin 2 (marker of surfactant secretory vesicles) and EEA1 (endosomal marker). Only significant p-values are shown.