Figure 6From: A non-BRICHOS surfactant protein c mutation disrupts epithelial cell function and intercellular signalingIntracellular lipid content and lipid secretion of MLE-12 cells expressing SP-CWT or SP-CI73T. (A) Intracellular lipid content of cells stably expressing WT SP-C or I73T mutant were quantified by mass spectrometry. Untreated cells (-) or cells treated with 10 Ī¼M methylprednisolone (+Met) or hydroxychloroquine (+Hyd) for 24 hours prior to sample isolation. Values were calculated as % of the mean of the untreated WT values. (B) after removal of detached cells, the lipids in the cell supernatant were analysed and presented as in (A). The graphs show relative amounts of phosphatidylcholine and lyso-phosphatidylcholine. Only significant p-values are depicted.Back to article page