Figure 3
From: Acetylated microtubules are required for fusion of autophagosomes with lysosomes
Impact of paclitaxel, vinblastine and nocodazole on microtubular acetylation and structure. (A) Immunostaining of microtubules in HeLa cells untreated or treated with 10 μM of nocodazole, vinblastine or paclitaxel overnight with antibodies against acetylated α-tubulin and normal β-tubulin. Ac-Tub, acetylated α-tubulin. β-Tub, β-Tubulin. Vin, vinblastine. (B) Immunoblotting analysis of acetylated α-tubulin levels in lysates of HeLa cells treated with different concentrations of nocodazole, vinblastine and paclitaxel for overnight.