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Figure 6 | BMC Cell Biology

Figure 6

From: The role of HIF-1 in up-regulating MICA expression on human renal proximal tubular epithelial cells during hypoxia/reoxygenation

Figure 6

Transduction of HIF-1alpha siRNA can inhibit surface expression of MICA on HK-2 cells during hypoxia/reoxygenation. The relative protein expression levels were determined by flow cytometry with a PE conjugated mouse anti-human MICA mAb 8 h after reoxygenation. The percentage of cells stained by MICA mAb is shown by the numbers in the top right of each graph. (A) HK-2 cultured in hypoxia (B) HK-2 treated with negative control siRNA, cultured in hypoxia (C) HK-2 treated with HIF-1alpha siRNA, cultured in hypoxia (D) HK-2 cultured in normoxia, no treatment (E) Isotype control.

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