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Figure 7 | BMC Cell Biology

Figure 7

From: The role of HIF-1 in up-regulating MICA expression on human renal proximal tubular epithelial cells during hypoxia/reoxygenation

Figure 7

NK cell cytotoxicity towards HK-2 cells was measured by LDH release assay kit. HK-2 cells were collected 96 h after transduction of Ad.CMV.HIF-1alpha, Ad.CMV.HIF-1alphaDELTAODD, Ad.CMV.LacZ and 8 h after hypoxia/reoxygenation with/without RNAi treatment. Before cells were used in the cytotoxicity assay, a blocking mAb was added or not. (A) No antibody, (B) isotype-matched mAb, (C) blocking MICA mAb. LDH measurements were made in triplicate from three separate assays. Values are shown as means ± SD from three independent experiments. *indicates a significant difference compared with the control group (p < 0.05).

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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