Cell viability and transformation assays. A. Cell viability of Primary and F111 cells. Cells were collected by trypsinization and plated on agar-coated surfaces at their represented time periods and assayed for viability by using MTT. Loss of cell viability is progressed with the time as shown in the figure with unfilled and filled bars representing Primary and F111 cells. B. Soft agar colony formation of primary and F111 cells. 1×105 cells collected at the indicated time periods were mixed with 0.3% of agar and overlaid on 1% agar. The colonies with more than 50 cells were accounted and the number was high at 16 h time period as indicated in the figure. Unfilled and filled bars in the figure represent Primary and F111 cells. C. Tumour formation assay in nude mice. Adherent for 16 hrs (A16) (crossed bars) and non-adherent for 16 hrs (NA16) (Filled bars) cells derived from F111 cells were injected subcutaneously in to nude mice and the size of the tumour increased with F111 NA16 cells was measured at indicated time periods and average size of the tumour is represented in the figure.