Cancer Stem cell marker analysis. Immunofluoroscence of different type of cells derived from F111 cell lines, representing A16 (a), NA16 (b), colony (c) and tumour (d) cells were stained with specific stem cell marker proteins such as ABCG 2 (Figure 5A) and CD133 (Figure 5B). The figure indicates the higher expression for both proteins in F111 NA16 cells. Magnification bars are included in the figure. Figure 5C Flow Cytometry analysis. FACS analysis for side population activity in A16, NA16, colony and tumour cells were done by staining with Hoechst dye (a, b, c, d successively) and their respective inhibition with verapamil treatment (e, f, g, h successively). The Side population community is represented in the gate and was high in F111 NA16 as shown in the figure.